| 质粒名称: | pSRE SSG |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 127692 |
| 存储实验室: | Justin English, Bryan Roth |
|
载体骨架基本信息 |
|
| 载体名称: | pSRE |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Sindbis Structural Genome |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSRE SSG was a gift from Justin English & Bryan Roth (Addgene plasmid # 127692 ; http://n2t.net/addgene:127692 ; RRID:Addgene_127692) |
| 参考文献部分: | VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells. English JG, Olsen RHJ, Lansu K, Patel M, White K, Cockrell AS, Singh D, Strachan RT, Wacker D, Roth BL. Cell. 2019 Jul 2. pii: S0092-8674(19)30622-1. doi: 10.1016/j.cell.2019.05.051. 10.1016/j.cell.2019.05.051 PubMed 31280962 |
买家导航
pSRESSG载体基本信息
pSRESSG载体图谱质粒图谱和多克隆位点信息
pSRESSG载体简介
pSRESSG载体序列
LOCUS pSRE SSG 8190 bp ds-DNA circular SYN 10-MAY-2023
DEFINITION Plasmid expresses the Sindbis Structural Genome (SSG) via the serum
response element (SRE) promoter. See Resource Information section
for annotated plasmid sequence..
ACCESSION .
VERSION .
KEYWORDS pSRE SSG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8190)
AUTHORS English JG, Olsen RHJ, Lansu K, Patel M, White K, Cockrell AS, Singh
D, Strachan RT, Wacker D, Roth BL
TITLE VEGAS as a Platform for Facile Directed Evolution in Mammalian
Cells.
JOURNAL Cell. 2019 Jul 2. pii: S0092-8674(19)30622-1. doi:
10.1016/j.cell.2019.05.051.
PUBMED 31280962
REFERENCE 2 (bases 1 to 8190)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 10, 2023 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8190
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(4077..4108)
/label=minP
/note="minimal TATA-box promoter with low basal activity"
misc_feature 4287..4378
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind complement(4319..4338)
/label=RVprimer3
/note="pGL3 vector, forward primer"
polyA_signal 4392..4440
/note="synthetic polyadenylation signal"
CDS 4545..5405
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5605..6193
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6094..6113
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6347..6364
/label=L4440
/note="L4440 vector, forward primer"
polyA_signal 6520..6568
/note="synthetic polyadenylation signal"
CDS complement(6592..7629)
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
EVGRV"
RBS 6605..6613
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
promoter complement(7660..8017)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7674..7809
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(7728..7747)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 7974..7994
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
1 tgttaaaaac aaaattttgt tgattaataa aagaaataat aaaaagttat gcagacgctg
61 catggcatta tgcaccatgc ttcctcagaa atacattgag ttttggcgtc cgctgaataa
121 atggttaata tagtgattat ttggcaacac tgcgcagcat tatgcactgc gcttcctcgg
181 aaatacgtcg agttttggtg ttgctatatt gcccgcggta agcggggatc taatatacca
241 gcctgatgca ttatgcacat cagttcctcg gaagtacatc gagttttgct ggtcgggtca
301 ttggggcgta gcggtcatct tcgtgtgcta gtcagcatca tgctgcaagc aaaaatcata
361 agtcctataa ttaatagcga cgaggcgccg ccgaaaaggg caaacagcca actccatgaa
421 gtttttgaga tggcggcttg gaattcttgg tcatttttgt gcggggtgct cacgatatgg
481 tcagctggtg gtttgcattc tgcattgcat gttgtcttct taccacacag cgatacaata
541 aagttcgcct gtgggctcgc ggtgctgaag tgtactgtca ccgctccttt ctccaggaca
601 tgaactgtcg actcttggag ggttgctgtg ctcgaatgcg aatgtacagg gcattgtcct
661 tcgcggtcgg atacatactg cagggtagcc atcccgccga agtccgctga gtaagtgcac
721 tcactgacat cacatttgac tgttgagacc agtggtgcat ctgatgtcct gataaaggca
781 gcgttcggga tgtcgataga gatgggaatg ttcccgtatg agcagtccac cgctcgaagc
841 ggattgactg caatcttgca cccgaaaggg gcggtttcct gcagtgggcg gcctgagttg
901 tttttccaca tctcgaatcc agatgcggcc tgcgtgtacg ggacatgcac gttcttggcg
961 gaaggcttga gtagtctaat gtctgtgctg gcgatgagat ctttgctagt caaggaggta
1021 gcttgaatgt ctccaaacgc tcctggtttc atcgctccgt attccgggaa gtcatagttg
1081 tacaccaggc cgcgatggat aacgaccttg tgatcgaatg gtgtaaacga tgctgaaatt
1141 ggtccagcta tgactttcag gtctttagac gttcctggtg tgactccgtt cacgtacaca
1201 tctaggaaac tggtagtgtt cccgtacact atacgtagtc ctactttcat cgcggcagta
1261 tgcaccttaa tcgcctgcgc gtggtcagtc gcgcaatctg ctgacaattc gacgtacgcc
1321 tcactcatct ggctgttctc actgtcgcaa aaacattgtg ctcctcccca catgaagggg
1381 tacacccctc caaagacctt gcaggtatag tctgcgtgag cggcgggctg acattccaag
1441 gagccgcagc atttgacttt aggggagggg accacagtgg tgaatttgca ggtgatgtac
1501 tcttggttgg tggaaggcaa aacctccgag gacatgacag taatctccaa attgagcggg
1561 gcgtaccctg ccctttcaac aagtgcctta tacggtatct gtggcacatt tggaacagtg
1621 gtcgcatgtt cgtaggcgtc taccttcgcc aggtaggcgc cggcaaccac taaaaaaggc
1681 aggcagcatg agcaacagcg cattagaacg atgacagcgg ccaggggtat acacagctgg
1741 acccagaaga atggctggct gttcgaccat aggtaactca tggtctcggt gaatgtttca
1801 gcattagccg acctaacaca gcacaaaagt gccagcgaag ttggaatcac ggcatttggg
1861 gccagggcat atggcgtcag gcactcacgg cgcgctttac aggcacataa tgctgcaaca
1921 gttacgccaa tcatcatcgc cacagcagct gatgcgacgg ctaagatggt gtacacagga
1981 tggcgatggt agtaatgctg tactatttcg tgtggccatc cgtgagggtc tcctggtgca
2041 gactcttggg catagaccct taccggttcg tgattgcccc atatgtattc caggccatct
2101 cggtcgacgg tgaagtttct aaccgtcttt ccgatgatcc attcagtagt tggttccgga
2161 tttgccccta gtctcctggt ggtgagcaat gtcaggtggt ctgtgtctaa ttggaggctg
2221 atgtgtttaa agccgtgtac tacgttcggc gcgtgggcaa cagggaccat gcaggtactc
2281 gggatcagct tgaaaggtaa atgcaatttc ccttgggccg tgtggtcggc atgtctgatc
2341 aagtccggcg aattgaagac ccacttcgtt tggtcgctct tataggcgac gcactgcttg
2401 atggcggtgc agcccgtgat ttcggtacgg gtcgtaacgg taccggtctt gtaatcgccg
2461 cacttgcact cgtacgtaat gttctttccg gatggtggct tcgcgtagac tttccctgat
2521 gattcctcca gataggacgt ataggcgtgc ggtcccggcc tgtgcatagt gatgtagccg
2581 gcggttgttt ctttcagacg gtcgtacact gtgcaaggaa tcttcttacc gtgaacggga
2641 ggtaggtcat atttttcccg tcccacgaat tttggtttta tcttgcgggc cattgtgcat
2701 gacgtcgctg agttgctact cgctatacta accgttacgc tgtcccctgg aggacacttc
2761 gcgaggagaa agtatccttt gtagctaagc cttctacacg gtcctgaggt gctgatcttg
2821 atgtcatcca tggtgccttc tttgacagta tgatcctgct cgagcgacat gtagcggtac
2881 ttatttgagc ttgctgctcc gctttggtcg tatccaaact gggcggaagt ctgtatgcgt
2941 atggtgttgt cgtccgcttc atcccagacc tgctcgatct taatcgggct aaagcacggt
3001 tcagtatggt gacagtacga gcatgtgccc aagtacgggc tggtcaaggt aaagtcgtca
3061 gtgacgcttc ttttacttct gccggacgat ccgcaccgca atatggcgtt gagcagggtg
3121 tcgtaggcct cgtggttcac gttctcttca aggatgtcaa gagctctgga tggttcgcgg
3181 gtgtagcatg tgggcgggcg attgcatggg aagctcacgt ttccaagcaa gcacatggcc
3241 gtgaccagtg gtgctgcaga ccactcttct gtcccttccg gggttgtctt gattgtcttc
3301 cctttgctat tccaggtgac gaccgaaagg gcagttcttg ttccctcatc agcccctccg
3361 aggactatcg cgacaacccg gcctgagtta tccataatcg gacgaccact gtctcctctg
3421 cctcctactc cgcgggggat ggtaaatcta cctccactat actgcaccgc tccgtggtgc
3481 cagttgtaaa acccttcagg gtgttcgctg gtgtaggtga acgcctcact tctcatgttg
3541 accggcaact gtgcgaactc catgtcgtat gctgacgact tggtgaattt gagctttgat
3601 agcacagggt ggtcaatagt tcctttcacg tggagtggtt tcattacctt tccttccatg
3661 gccagtgcgt gcccgatgac atctccgtcc tcatttttga cgtcgaacag tctgtcggcc
3721 tccaacttga gtgccatacg ttgtctcttt ccgggtttgg gttttgcagg ttgcttcttc
3781 ttcttctcct gtgtttttgg tttcttcggc ttcggtggtt gctttggcgc ctgcttcttc
3841 tggcgcggcg gcgggcgtgg gcgtggggtt tgaggtctag ttgcctgtcc aatgactagg
3901 gcactgacgg ctgtggtcag ttgctggatt tgggaagcca gcccattgcg ggcaggcatc
3961 ggggccgcct gcctccttct ccgcggcctc cacatggcag tgggggccgg gaaggggcgg
4021 cggccgagca tgttaaagaa tcctctattc atggatccca gtaccggatt gccaagctgg
4081 aagtcgagct tccattatat accctctagt gtctaagctt ggccgccgag gccagatcta
4141 gatgtcctaa tatggacatc ctagatgtcc taatatggac atcctagatg tcctaatatg
4201 gacatcctag atgtcctaat atggacatcc tagatgtcct aatatggaca tcctgctagc
4261 gagctcaggt accggccagt taggccagag aaatgttctg gcacctgcac ttgcactggg
4321 gacagcctat tttgctagtt tgttttgttt cgttttgttt tgatggagag cgtatgttag
4381 tactatcgat tcacacaaaa aaccaacaca cagatgtaat gaaaataaag atattttatt
4441 gcggcctgtc caatacctcc cgtaccttaa tattacttac ttatccttga gagacgtact
4501 agtaaccctg ataaatgctt caataatatt gaaaaaggac gagtatgagc atccaacatt
4561 ttcgtgtcgc actcattccc ttctttgcgg cattttgctt gcctgttttt gcacaccccg
4621 aaacgctggt gaaagtaaaa gatgctgaag atcaactggg tgcaagagtg ggctatatcg
4681 aactggatct caatagcggc aagatccttg agtctttccg ccccgaagaa cgattcccga
4741 tgatgagcac tttcaaagta ctgctatgtg gcgcggtgtt gtcccgtata gacgccgggc
4801 aagagcagct tggtcgccgt atacactact cacaaaacga cttggttgag tactcgccgg
4861 tcacggaaaa gcatcttacg gatggcatga cggtaagaga attgtgtagt gctgccatta
4921 ccatgagcga caacaccgcg gccaacttac ttctgacaac gatcggaggc cctaaggagc
4981 tgactgcatt tcttcataat atgggtgatc atgtgacccg gcttgaccgc tgggaaccag
5041 agttgaacga agccataccg aacgacgagc gtgataccac gatgccagta gcaatggcca
5101 caactcttcg gaaactactc actggcgaac ttcttactct agcatcacga cagcagctca
5161 tagactggat ggaggcggac aaagtagcag gaccacttct tcgctcggcc ctccctgctg
5221 gctggttcat tgctgacaaa tcgggggccg gtgaacgcgg ctctcgcggc atcattgctg
5281 cgctggggcc tgatggtaag ccctcacgaa tcgtagtgat ctacacgacg gggagtcagg
5341 ccactatgga cgaacgaaat aggcagatcg ctgagatcgg tgcctcactg atcaagcact
5401 ggtaaccact gcagtggttt agcatttgcg gccgctgtca gaccaagttt actcatatat
5461 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
5521 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
5581 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
5641 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
5701 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
5761 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
5821 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
5881 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
5941 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6001 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6061 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6121 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6181 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6241 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
6301 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
6361 cgaggaagcg gaagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag
6421 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
6481 aggctctcaa gggcatcggt cgacagcatc tagttcgaac acacaaaaaa ccaacacaca
6541 gatgtaatga aaataaagat attttattgc ggccatgacc ggttctagag tttaaactcg
6601 acctacctcc ttagcgcgcg gtcgtgtact gggccggcgg ttgccgctgt cggccagcac
6661 ctcgacgcag ccgtcggtcc atacggctgc gctccggcga gcgatttgtg tgcgaccgac
6721 ggtgccggcc ccgctgcgga cgatggcatc gcagcggcct tgagcccagg cagcatcgtc
6781 gaagttgccg tccacgaggc tctggtagag ctgatccagg ccgatgcgca gcatgtaggc
6841 tcgcagacga gggctgccgg ccagctcggg atgccggcgc tcgaagtagc gagtctgctg
6901 ctccatgcaa gccagccagg gccgccagaa gaagatgttg gccacctcgt actgactgtc
6961 cccgaacata gcttcggacc agtcgattac ggcggtgatg cggccgttgt cggtcaggac
7021 gttgttgctg ccgaagtcgg catggacgag gtggcgcacc tcgggacagt cttcggccca
7081 cagcatcagt tcgtccaggg cttgagctac gctggcggac acggtgtcgt ccatcacggt
7141 ctgccagtgg tagacatggg gatcagcaat ggcgcaaatg aaatcccgcc aggttgtgta
7201 ctggccgatg ccttggggcc cgaatgggcc gaagccgctg gtttgactga ggtcggcggc
7261 ggcaatagca tccatagctt cggcgacagg ctgtaacaca gcaggcagct ctgtttcggg
7321 gaggtcttgg agagtaacgc cttgggcgcg tctactgatg cagtatgtca ggctctcgct
7381 gaactcgccg atgtccaaca cttcggggat gggtagtgca gcgctggcga agtggcggta
7441 cacgtagcgg tctttgtaga agccatcagc gcagctattc acccgcagta catagccgcg
7501 tccgccgaca tcgaagctga aggctcggct ctcttcgccc tccgacaact gcatcaggtc
7561 gctcacactg tcgaacttct cgatgagaaa tttttcaacg ctggtagcgg tgagttcggg
7621 cttcttcatg gtggcgctag tgtcagaaga atcgagcttt ttgcaaaagc ctaggcctcc
7681 aaaaaagcct cctcactact tctggaatag ctcagaggca gaggcggcct cggcctctgc
7741 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
7801 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
7861 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
7921 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
7981 ctgacacaca ttccacagct ggttctttcc gcctcagaag gtagctaacc aagttcctct
8041 ttcagaggtt atttcaggcc atggtgctgc gcagatcagc ggaagagcgc cccccgggca
8101 aataggggtt ccgcgcacat ttccccgaaa agtgccacct ggccctttcg tcttcaagaa
8161 ttaattcccc tcgagctcta gagtttaaac
//
